Refinement of Minipig Surgical Procedures
Sydney Mensen2022-12-20T07:54:34-06:00The importance of refinement does not need to be discussed; however, it is equally important to share the advances made with the community. At times individual refinements might be small and of little impact but over time they add up and improve a procedure considerably. In this presentation the advancements of surgical procedures performed in Göttingen Minipigs over the last few years are summed up and discussed. The following procedures are addressed: LVP Telemetry by left sided Thoracotomy: This procedure has ben refined in a way that with larger Minipigs only one incision is necessary. With other adjustments we brought down [...]
Surgical Anesthetic Preparation in the Juvenile NHP
Sydney Mensen2022-09-27T10:16:23-05:00Due to the high demand for NHP’s as research models, and the disruptions that Covid 19 created amongst the suppliers of NHP’s, we have been dealing with a downward trend in the range of body weights in our surgical animals. 4 to 5 years ago, the average size of an NHP that we would be performing a surgical procedure on was around 3.5 kgs. Now, we are routinely performing major surgical procedures on animals from 1.5 kgs to 2.5 kgs. These smaller animals are technically juveniles and they can have different anesthetic needs. This change in size of the animal can [...]
Cerebral Oxygenation Monitoring in Rats Under Isoflurane Anesthesia
Sydney Mensen2022-09-27T10:16:23-05:00Introduction: Although monitoring of vital signs in small lab animals is critical to successful anesthetic outcomes, reliable measurement of the vital signs is challenging due to their size. To this end, we are developing a miniaturized optical imaging device based on the Near Infrared Spectroscopy (NIRS) technique for noninvasive measurement of the brain and peripheral tissue oxygenation and perfusion. Our goal is to develop a device to support safe and reliable anesthesia monitoring in small lab animals to improve animal welfare, ensure successful outcomes, and create reproducible research, which will ultimately aide in better knowledge of the living systems to enhance [...]
Aseptic Surgery in Rodents; Fact or Fiction
Sydney Mensen2022-09-27T10:16:23-05:00Surgery The surgical procedure is often referred to in terms of surgery, but it should be referred to as a surgical act that is made up of: patient preparation, equipment preparation, preparation of the surgeon, surgical procedure, post-operative care and follow-up Surgical asepsis any measure taken to reduce bacterial contamination at the surgical site, aiming to reduce postoperative infections. • Direct measures • Patient preparation: shaving, depilation, disinfection of the surgical site, sterile drapes, sterile envelope for instruments, continuity of the operating field, normothermia, hydration, analgesia • Equipment preparation: sterile, adapted, back up kit? • Preparation of the surgeon • Indirect [...]
Wet Lab #6: Rodent Intubation – Afternoon Session
Sarah McFarlane2022-09-27T10:17:16-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #5: Rodent Intubation – Morning Session
Sarah McFarlane2022-09-27T10:16:44-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #2: Introduction to Minimally Invasive Surgical Techniques in Swine – Afternoon Session
Sarah McFarlane2022-09-27T10:17:16-05:00This amazing facility provides us with the opportunity to instruct on minimally invasive surgical techniques in the swine model. Students will get the opportunity to perform endoscopic, laparoscopic, and fluoroscopy-guided endovascular procedures. It is not very often that we have had the opportunity to provide instruction using fluoroscopy and we are very excited about this lab! Anesthesia, proper handling of the equipment and instruments needed to perform these procedures will be mentored, along with specific techniques and tips for each. This wet lab would not be possible without the generous donation from Premier BioSource. To learn more about their new barrier [...]
Wet Lab #1: Introduction to Minimally Invasive Surgical Techniques in Swine – Morning Session
Sarah McFarlane2022-09-27T10:17:16-05:00This amazing facility provides us with the opportunity to instruct on minimally invasive surgical techniques in the swine model. Students will get the opportunity to perform endoscopic, laparoscopic, and fluoroscopy-guided endovascular procedures. It is not very often that we have had the opportunity to provide instruction using fluoroscopy and we are very excited about this lab! Anesthesia, proper handling of the equipment and instruments needed to perform these procedures will be mentored, along with specific techniques and tips for each. This wet lab would not be possible without the generous donation from Premier BioSource. To learn more about their new barrier [...]