Afternoon Wet Lab – Vanderbilt University
Christine Siebert2024-03-18T04:28:34-05:00Intermediate swine procedures and rat microsurgery and catheterization techniques
Curious George Goes to The Podium
Christine Siebert2024-03-18T04:28:35-05:00Nose Cone vs Intubated Ventilation During Open Chest Surgery in Rats – Nina Krutrök, AstraZeneca The aim of this study was to investigate if nose cone ventilation is comparable to endotracheal intubation. The latter is the most common approach for air delivery to rats undergoing open chest surgery for cardiovascular research during for example induction of myocardial infarction or epicardial injections. This method of air delivery is technically challenging leading to prolonged procedures and increased risk of endotracheal complications such as laryngeal damage and laryngeal edema, oral pharyngeal and/or tracheal laceration due to multiple intubation attempts. Nose cone ventilation is a [...]
Morning Wet Lab – Vanderbilt University
Christine Siebert2024-03-18T04:28:36-05:00Basic swine laparoscopy and endovascular interventions paired with an introduction to stereotaxic, intrathecal surgery, and vascular catheterization in the rat
Development of a Novel Technique for Ambulatory Left Ventricular Pressure Measurement in Mice
Christine Siebert2022-09-27T10:16:21-05:00Introduction: Industry has seen a shift in the use of genetically modified mice for the evaluation of cardiac performance and function. There is significant interest in developing novel therapies to treat heart failure and measuring the effectiveness of compounds. Many models exist in larger species however, there are limited translational models for mice. To evaluate compounds in mice, often large animal models are scaled down to be utilized in studies. In this presentation we will discuss the development of a murine model of left ventricular pressure cannulation (LVP). Currently, echocardiography is routinely used to assess disease progression in rodent models of [...]
Cerebral Oxygenation Monitoring in Rats Under Isoflurane Anesthesia
Christine Siebert2022-09-27T10:16:23-05:00Introduction: Although monitoring of vital signs in small lab animals is critical to successful anesthetic outcomes, reliable measurement of the vital signs is challenging due to their size. To this end, we are developing a miniaturized optical imaging device based on the Near Infrared Spectroscopy (NIRS) technique for noninvasive measurement of the brain and peripheral tissue oxygenation and perfusion. Our goal is to develop a device to support safe and reliable anesthesia monitoring in small lab animals to improve animal welfare, ensure successful outcomes, and create reproducible research, which will ultimately aide in better knowledge of the living systems to enhance [...]
Aseptic Surgery in Rodents; Fact or Fiction
Christine Siebert2022-09-27T10:16:23-05:00Surgery The surgical procedure is often referred to in terms of surgery, but it should be referred to as a surgical act that is made up of: patient preparation, equipment preparation, preparation of the surgeon, surgical procedure, post-operative care and follow-up Surgical asepsis any measure taken to reduce bacterial contamination at the surgical site, aiming to reduce postoperative infections. • Direct measures • Patient preparation: shaving, depilation, disinfection of the surgical site, sterile drapes, sterile envelope for instruments, continuity of the operating field, normothermia, hydration, analgesia • Equipment preparation: sterile, adapted, back up kit? • Preparation of the surgeon • Indirect [...]
Wet Lab #6: Rodent Intubation – Afternoon Session
Christine Siebert2022-09-27T10:17:16-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #5: Rodent Intubation – Morning Session
Christine Siebert2022-09-27T10:16:44-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #4: Microsurgical Techniques and Vascular Catheterization in the Mouse – Afternoon Session
Christine Siebert2022-09-27T10:17:16-05:00This lab will provide training on vascular catheterization in rodents in a unique and state of the art facility with talented instructors. In addition to sharing surgical tips and tricks for success, our instructors will also review updates to surgical and anesthetic processes, surgical instruments, and post-operative recommendations for achieving long-term patency in catheterized rodents. The wet labs will also focus on the use of the Instech Vascular Access Button where applicable. Course Fee – $600.00 per session Wet Lab #4 – Afternoon Session Mouse (Max 16 persons) Wed., September 14, 2022 01:00pm – 05:00pm
Wet Lab #3: Microsurgical Techniques and Vascular Catheterization in the Rat – Morning Session
Christine Siebert2022-09-27T10:17:16-05:00This lab will provide training on vascular catheterization in rodents in a unique and state of the art facility with talented instructors. In addition to sharing surgical tips and tricks for success, our instructors will also review updates to surgical and anesthetic processes, surgical instruments, and post-operative recommendations for achieving long-term patency in catheterized rodents. The wet labs will also focus on the use of the Instech Vascular Access Button where applicable. Course Fee – $600.00 per session Wet Lab #3 – Morning Session Rat (Max 16 persons) Wed., September 14, 2022 08:00am – 12:00pm