Nose Cone vs Intubated Ventilation During Open Chest Surgery in Rats – Nina Krutrök, AstraZeneca The aim of this study was to investigate if nose cone ventilation is comparable to endotracheal intubation. The latter is the most common approach for air delivery to rats undergoing open chest surgery for cardiovascular research during for example induction of myocardial infarction or epicardial injections. This method of air delivery is technically challenging leading to prolonged procedures and increased risk of endotracheal complications such as laryngeal damage and laryngeal edema, oral pharyngeal and/or tracheal laceration due to multiple intubation attempts. Nose cone ventilation is a [...]
Practical Approach to Pain Management for Nonhuman Primates: Enhancing Welfare and Research OutcomesSydney Mensen2023-10-30T09:07:18-05:00
Speaker - Jan Bernal Amgen Pain management is a critical component of responsible animal research and care, particularly in the context of nonhuman primates (NHPs) utilized in biomedical and behavioral studies. This presentation aims to provide a comprehensive overview of practical pain management strategies for NHPs, with a focus on enhancing their welfare while optimizing research outcomes. The presentation will begin by defining pain and classifications of pain. It will emphasize the pain pathways and the multimodal approaches effective at each of the pain pathways, encompassing a wide range of medication and procedural options to modulate pain. Attendees will gain [...]
Speaker - Kimicia Isaac, St. Kitts Biomedical Research Foundation / Virscio, Inc. The NIH estimates 800,000 Americans suffer strokes yearly, with significant mortality, long-term disability, and substantial unmet medical need. The lack of translation from small animal models to clinical efficacy has slowed therapeutic development. Nonhuman primates (NHP) share anatomy and physiology with humans important to the clinical pathophysiology of stroke and truly predictive preclinical modeling. An model of transient ischemia is described in adult male African green monkeys (Chlorocebus sabaeus) that promises to enable more robust evaluation of stroke therapeutics. Methods: 11 monkeys received a 180 min occlusion of [...]
This presentation will review the phases of wound healing, physiologic steps to achieve proper healing, the application of the graft as a wound dressing and the use of antimicrobial hydrogel, Manuka honey and foam dressing in combination with the graft as well as examples of wounds, difficulties encountered, successes achieved and summary of histological examination of tissues biopsied during this process.
Auditory related health conditions have warranted the development of new treatments and drugs including, for example, gene therapy focusing on restoring the sensory receptors of the auditory and vestibular systems known as hair cells. However, accessing and treating the cochlea containing these hair cells offers a formidable surgical challenge. This presentation will introduce anatomical features, landmarks, and dosing techniques for the cochlea. Surgical hints are shared but also pitfalls that may be encountered when developing and performing these dosing procedures are also discussed.
Lumbar intrathecal access port implantation for Cerebrospinal fluid (CSF) collection and/or intrathecal infusion in the non-human primate is being requested more frequently in the Safety Assessment world of biomedical research. Historically, performing a hemi-laminectomy to visualize and access the spinal dura for catheter cannulation has been the gold standard for the placement of these access catheters. There is an alternative. A minimally invasive technique using a Tuohy needle to access the intrathecal space and the advancing of an access catheter through this needle into the IT space with the need to perform a hemi-laminectomy, has proven to be an advantageous technique [...]
Introduction: Methadone, a synthetic opioid has gained popularity as an analgesic for surgical procedures as it provides a longer effective duration compared to common opioids for moderate to severe pain. Pharmacokinetic (PK) data in dogs and humans demonstrate therapeutic duration for 12-14 hours. This study investigates the pharmacokinetics of methadone at 2 dose levels in Cynomolgus Macaques for use as extended duration opioid for surgical procedures involving moderate to severe pain post-operatively. Methods: 8 Mauritius origin Cynomolgus Macaques were administered 0.2mg/kg and 0.5 mg/kg methadone hydrochloride intramuscularly (IM) and plasma and urine collected at predetermined time points and analyzed. Average mean [...]
Due to the high demand for NHP’s as research models, and the disruptions that Covid 19 created amongst the suppliers of NHP’s, we have been dealing with a downward trend in the range of body weights in our surgical animals. 4 to 5 years ago, the average size of an NHP that we would be performing a surgical procedure on was around 3.5 kgs. Now, we are routinely performing major surgical procedures on animals from 1.5 kgs to 2.5 kgs. These smaller animals are technically juveniles and they can have different anesthetic needs. This change in size of the animal can [...]
Introduction: About 41,260 new cases (28,600 in men and 12,660 in women) over liver cancer will be diagnosed in 2022 according to the American Cancer Society website. A model resecting approximately 60% of the liver by weight in cynomolgus monkeys is valuable in evaluating potential therapies that encourage natural regrowth of the liver post resection in human patients. Methods: Spanning three separate studies, a laparotomy was performed on a total of 26 animals, resecting approximately 60% of the total liver volume (by weight) of each animal’s liver. Liver volume was calculated based on historical organ (liver) weights measured at necropsy, determined [...]