Anesthetic and Surgical Considerations for Liver Bleeding Assessment Models – Part II
Christine Siebert2024-03-18T04:28:34-05:00Speaker - Stephanie Vaughan Labcorp Early Development
Anesthetic and Surgical Considerations for Liver Bleeding Assessment Models – Part I
Christine Siebert2024-03-18T04:28:34-05:00Speaker - Stephanie Vaughan Labcorp Early Development
The Sheep as a Model for Assessing Long Term Functional Patency and Maintenance of Arteriovenous Grafts
Christine Siebert2024-03-18T04:28:35-05:00Speaker - John Carney University of Minnesota Patients with end stage renal disease often require vascular grafts to create arteriovenous (AV) shunts for hemodialysis access. However, graft complications frequently occur resulting in loss of functional patency and need for subsequent interventions. Although sheep have been used to evaluate preclinical graft safety, research lacks regarding use for prolonged graft maintenance and postoperative interventions. We sought to validate the sheep model in evaluating chronic AV graft patency and salvage techniques using clinically established approaches. Methods: Methods: Three (n = 3) adult sheep weighing 88 ± 21kg and 14 ± 1 months old [...]
Evaluation of an African Green Monkey Model of Transient Ischemia
Christine Siebert2024-03-18T04:28:35-05:00Speaker - Kimicia Isaac, St. Kitts Biomedical Research Foundation / Virscio, Inc. The NIH estimates 800,000 Americans suffer strokes yearly, with significant mortality, long-term disability, and substantial unmet medical need. The lack of translation from small animal models to clinical efficacy has slowed therapeutic development. Nonhuman primates (NHP) share anatomy and physiology with humans important to the clinical pathophysiology of stroke and truly predictive preclinical modeling. An model of transient ischemia is described in adult male African green monkeys (Chlorocebus sabaeus) that promises to enable more robust evaluation of stroke therapeutics. Methods: 11 monkeys received a 180 min occlusion of [...]
Automated Blood Sampling in a Telemetry Cardiovascular Dog Colony
Christine Siebert2024-03-18T04:28:36-05:00Speaker - Amanda Wilsey Abbvie Implementation of Automated Blood Sampling (ABS) allows for uninterrupted cardiovascular data collection with simultaneous blood collection throughout a telemetry dog preclinical study. This reduction shortens study timelines, minimizes CV data artifacts, and considerably reduces compound synthesis and the associated costs. Methods: A colony of telemetry instrumented male beagle dogs were implanted and maintained in-house for non-GLP preclinical cardiovascular safety studies. Evaluation of the ABS telemetry dog cardiovascular model; highlighting the benefits and downfalls of the last seven years since implementing Automated Blood Sampling. Results: Since 2016, telemetry dogs implanted with transmitters and vascular access ports [...]
Ameroid Band Constrictor-Induced Arterial Occlusion Simulates Peripheral Vascular Disease in a Porcine Model
Christine Siebert2022-09-27T10:16:24-05:00Introduction: Gradually tightening vascular occluders such as ameroid constrictors are used to model chronic ischemia and may recapitulate human ischemic disease pathophysiology and atherosclerotic plaque progression better than acute ligation. Ultrasonic power Doppler (US-PD) imaging uses Doppler sampling and filtering techniques that significantly increase the sensitivity and specificity of routine sonographic instruments for imaging spatially disorganized patterns of peripheral perfusion without contrast enhancement. Objective: To compare sensitivity of US-PD with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) techniques for measuring endothelial function and determining vascular perfusion. Methods: Four 6-month-old, castrated male, Yucatan minipigs were operated. A~9 cm incision was made in the [...]
Baseline Hemodynamics Including Aortic and Pulmonary Blood Flow in a Chronic Bovine Model
Christine Siebert2022-09-23T14:44:57-05:00The use of large animal models in preclinical studies has provided invaluable data for evaluating the safety and efficacy of new therapies and devices and has facilitated the development and clinical application of cardiovascular innovations. With an increasingly valuable role, the implementation of large animal models has expanded, serving to bridge the translation of physiologic and economic aspects of animal research to the successful treatment of human heart failure. In this study, we report normal hemodynamic values, including aortic and pulmonary blood flow, in a bovine model at rest and during exercise after a control procedure.