Morning Wet Lab – Vanderbilt University
Christine Siebert2024-03-18T04:28:36-05:00Basic swine laparoscopy and endovascular interventions paired with an introduction to stereotaxic, intrathecal surgery, and vascular catheterization in the rat
Surgery in Zoo and Wildlife Settings: From the Outback to Balboa Park
Christine Siebert2022-09-27T10:16:21-05:00Surgeries in Zoo and Wildlife settings present unique challenges, from a wide array of anatomic and physical challenges to the n=1 conundrum. Dr. Reed from the San Diego Zoo will share cases she has encountered during her career in Australian wildlife hospitals, aquaria, and now in a large American zoo. With an emphasis on avian orthopedic fracture repair, approaches to surgery and post operative care in non-traditional species (including invertebrates, fish, large carnivores, and megavertebrates) will be discussed.
Development of a Novel Technique for Ambulatory Left Ventricular Pressure Measurement in Mice
Christine Siebert2022-09-27T10:16:21-05:00Introduction: Industry has seen a shift in the use of genetically modified mice for the evaluation of cardiac performance and function. There is significant interest in developing novel therapies to treat heart failure and measuring the effectiveness of compounds. Many models exist in larger species however, there are limited translational models for mice. To evaluate compounds in mice, often large animal models are scaled down to be utilized in studies. In this presentation we will discuss the development of a murine model of left ventricular pressure cannulation (LVP). Currently, echocardiography is routinely used to assess disease progression in rodent models of [...]
Technical Insights for Cochlear Administration in the NHP
Christine Siebert2022-09-27T10:16:22-05:00Auditory related health conditions have warranted the development of new treatments and drugs including, for example, gene therapy focusing on restoring the sensory receptors of the auditory and vestibular systems known as hair cells. However, accessing and treating the cochlea containing these hair cells offers a formidable surgical challenge. This presentation will introduce anatomical features, landmarks, and dosing techniques for the cochlea. Surgical hints are shared but also pitfalls that may be encountered when developing and performing these dosing procedures are also discussed.
Refinement of Minipig Surgical Procedures
Christine Siebert2022-12-20T07:54:34-06:00The importance of refinement does not need to be discussed; however, it is equally important to share the advances made with the community. At times individual refinements might be small and of little impact but over time they add up and improve a procedure considerably. In this presentation the advancements of surgical procedures performed in Göttingen Minipigs over the last few years are summed up and discussed. The following procedures are addressed: LVP Telemetry by left sided Thoracotomy: This procedure has ben refined in a way that with larger Minipigs only one incision is necessary. With other adjustments we brought down [...]
Aseptic Surgery in Rodents; Fact or Fiction
Christine Siebert2022-09-27T10:16:23-05:00Surgery The surgical procedure is often referred to in terms of surgery, but it should be referred to as a surgical act that is made up of: patient preparation, equipment preparation, preparation of the surgeon, surgical procedure, post-operative care and follow-up Surgical asepsis any measure taken to reduce bacterial contamination at the surgical site, aiming to reduce postoperative infections. • Direct measures • Patient preparation: shaving, depilation, disinfection of the surgical site, sterile drapes, sterile envelope for instruments, continuity of the operating field, normothermia, hydration, analgesia • Equipment preparation: sterile, adapted, back up kit? • Preparation of the surgeon • Indirect [...]
Wet Lab #6: Rodent Intubation – Afternoon Session
Christine Siebert2022-09-27T10:17:16-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #5: Rodent Intubation – Morning Session
Christine Siebert2022-09-27T10:16:44-05:00In this wet lab we will be covering intubation of the mouse and rat. We will utilize Kent Scientific’s endotracheal kits including their intubation stands, fiber optic lights, vaporizers, and ventilators. Students can expect to learn identification of the appropriate anatomy, suitable supplies, and equipment for rodent intubation. In addition to this, we will share several approaches along with troubleshooting tips and tricks learned over many years of trial and error. This wet lab is a great opportunity to add this very challenging and highly desired technique to your resume. Mastering of rodent intubation will allow the expansion of you and [...]
Wet Lab #4: Microsurgical Techniques and Vascular Catheterization in the Mouse – Afternoon Session
Christine Siebert2022-09-27T10:17:16-05:00This lab will provide training on vascular catheterization in rodents in a unique and state of the art facility with talented instructors. In addition to sharing surgical tips and tricks for success, our instructors will also review updates to surgical and anesthetic processes, surgical instruments, and post-operative recommendations for achieving long-term patency in catheterized rodents. The wet labs will also focus on the use of the Instech Vascular Access Button where applicable. Course Fee – $600.00 per session Wet Lab #4 – Afternoon Session Mouse (Max 16 persons) Wed., September 14, 2022 01:00pm – 05:00pm