Speaker - Amanda Wilsey Abbvie Implementation of Automated Blood Sampling (ABS) allows for uninterrupted cardiovascular data collection with simultaneous blood collection throughout a telemetry dog preclinical study. This reduction shortens study timelines, minimizes CV data artifacts, and considerably reduces compound synthesis and the associated costs. Methods: A colony of telemetry instrumented male beagle dogs were implanted and maintained in-house for non-GLP preclinical cardiovascular safety studies. Evaluation of the ABS telemetry dog cardiovascular model; highlighting the benefits and downfalls of the last seven years since implementing Automated Blood Sampling. Results: Since 2016, telemetry dogs implanted with transmitters and vascular access ports [...]
Unbreak My Heart – The Reality of the Future? Regenerative Cardiovascular Research with Focus on Minipig ModelSydney Mensen2023-10-30T08:45:52-05:00
Speaker - Nina Krutrök AstraZeneca After myocardial infarction (MI) patients lose up to 1 billion cardiomyocytes. The inability of the injured mammalian heart to regenerate the loss of contractile myocardial tissue leads to ischemic heart failure (HF). Despite major advances in the treatment of HF, the root cause of impairment is still unaddressed. Human Ventricular Progenitor cells (HVPs) engraft, differentiate into cardiomyocytes, and prevent disease progression after -MI-in the murine and porcine heart.1, 2 In our research we aim to study the effect of HVPs as a new modality in a post-MI pig model. Methods: Myocardial infarction was induced using [...]
Introduction: Industry has seen a shift in the use of genetically modified mice for the evaluation of cardiac performance and function. There is significant interest in developing novel therapies to treat heart failure and measuring the effectiveness of compounds. Many models exist in larger species however, there are limited translational models for mice. To evaluate compounds in mice, often large animal models are scaled down to be utilized in studies. In this presentation we will discuss the development of a murine model of left ventricular pressure cannulation (LVP). Currently, echocardiography is routinely used to assess disease progression in rodent models of [...]
Introduction: Routine cardiac catheterization for angioplasty and stenting has created a large population of patients with cardiac damage and subsequent long-term heart failure. Cardiac therapies are often administered directly to the heart by methods such as coronary sinus retrograde infusion. Understanding the differences in human and swine coronary sinus anatomy is crucial in preclinical safety studies, particularly for biodistribution evaluation. Contrast/ Dye studies aid in determining coronary sinus anatomy and catheter placement in the heart. Methods: Under general anesthesia, 9f or greater sheath was placed via jugular vein cutdown on three Landrace/ Duroc and two Yucatan swine. Using fluoroscopic guidance, a [...]
The use of large animal models in preclinical studies has provided invaluable data for evaluating the safety and efficacy of new therapies and devices and has facilitated the development and clinical application of cardiovascular innovations. With an increasingly valuable role, the implementation of large animal models has expanded, serving to bridge the translation of physiologic and economic aspects of animal research to the successful treatment of human heart failure. In this study, we report normal hemodynamic values, including aortic and pulmonary blood flow, in a bovine model at rest and during exercise after a control procedure.